Fig. 4: Localization of E3 ligases and POI in HEK293T and mesothelioma model cell lines.
From: Expanding the toolbox to develop IAP-based degraders of TEAD transcription factors
a Immunoblots of equivalent total cell lysate (L), cytosolic fraction (C) and nuclear fraction (N) from indicated cell lines were probed for nuclear marker (Lamin B1), cytosolic marker (Hsp90), E3 ligases (cIAP1 and XIAP) and target proteins (TEAD1 and TEAD4). b Subcellular profiling of endogenous TEAD1 and cIAP1 degradation. NCI-H2052 cells were treated with dose titration of ALP hit A531 (four concentrations with 10-fold serial dilutions from 10 µM and DMSO vehicle control), nuclear and cytosolic fractions purified, and equivalent amounts run on Western blot. Antibodies against nuclear marker (Lamin B1), cytosolic marker (Hsp90), target protein (TEAD1) and E3 ligases (cIAP1 and XIAP) were used for probing the blots. All subcellular fraction experiments are performed as a single biological experiment (n = 1), but have at least n = 2 biologically independent experiments overall for TEAD1 and cIAP1/XIAP localization in NCI-H2052 cells (a, b). All uncropped blot images are available in Supplementary Data 1.
