Fig. 7: Analysis of TEAD-based IAP degrader activity on proliferation and transcription.
From: Expanding the toolbox to develop IAP-based degraders of TEAD transcription factors
a Viability of Hippo pathway-dependent mesothelioma cells ZL55, NCI-H226, NCI-H2052 and Hippo pathway-independent NCI-H520 cells was assessed post dose titrations of indicated IPDs for 7 days. IC50 (plotted as bars) and maximum inhibition % Emax (plotted as dot symbols) values were calculated from dose response curves (see Supplementary Data 1) for indicated cell lines. Data represent mean ± SD of n = 4 biologically independent experiments. b Effect of IPDs on transcript levels of TEAD-dependent genes. qPCR analysis of CTGF expression level (top panel) from indicated cell lines treated with 5 concentration points (1:10 dilution factor from 10 µM and DMSO) for 48 h. IC50 and Emax values were calculated as mean ± SD of n = 3 biologically independent experiments (n = 2 in the case of ZL55 cells). IC50 values marked >1000 and >10000 nM indicate the upper concentration limit for dose response fitting, as detailed in “Materials and Methods”.
