Extended Data Fig. 3: Transcriptional reprogramming in response to early-onset DR and late-onset AL.
From: A nutritional memory effect counteracts the benefits of dietary restriction in old mice
a, Heatmap of unsupervised clustering of expression changes for ALDR switch-resistant genes in BAT (n = 3–5 per group; colour bar represents z score range). b, Boxplot representation of scaled expression levels of differentially up- and downregulated genes under chronic DR as opposed to chronic AL controls in livers of ALDR switch mice at a young age. Whiskers represent 1st and 5th quartiles, box edges represent 2nd and 4th quartiles, and the centre line represents the 3rd quartile/median. c,d, Heatmap of expression changes for ALDR switch-resistant genes in BAT (c) and WAT (d) of young ALDR switch mice (n = 3 per group; colour bar represents z score range). Biologically independent animals used for RNA-seq: Liver: n = 3 (AL, DR, ALDR, DRAL, ALDR 5m, AL 5m); BAT: n = 3 (AL, DR, DRAL, AL 5m, ALDR 5m) n = 5 (ALDR); WAT: n = 3 (AL, DR, ALDR, AL 5m) n = 5 (ALDR, DRAL).
