Extended Data Fig. 7: Anti-cancer effect of cardiac glycosides across different human cancer cell lines.
a–b, Quantification of cell survival by trypan blue staining of Huh7 cells (a) and HLF cells (b) after treatment with the indicated drug combinations (n = 3). Timeline of the experiment is shown in Supplementary Fig 4a. Statistical significance was calculated using unpaired two-tailed Student’s t-test. c, Quantification of cell survival of senescent (alisertib, palbociclib) and control (DMSO) SK-Mel-5 melanoma cells (n = 4). Statistical significance was calculated using two-way ANOVA (Dunnett’s test). d, Quantification of cell survival of senescent (doxorubicin, palbociclib) and control (DMSO) MCF7 or MCF7 breast cancer cells infected with a shRNA against TP53 (n = 4). Statistical significance was calculated using two-way ANOVA (Dunnett’s test). e–f, mRNA expression levels of TP53 in MCF7 cells (e) and HCT116 cells (n = 3). Statistical significance was calculated using unpaired two-tailed, Student’s t-test. Data represent mean ± s.d; n represents independent experiments; ns, not significant.
