Extended Data Fig. 9: Ouabain treatment resets immune infiltration in old mice.
a, xCell analysis of the transcriptome data predicts changes in immune infiltration in the liver of old mice that could be reverted with ouabain. RNA-Seq data from the livers of young (n = 6) and old mice, either treated with vehicle (n = 6) or ouabain (n = 6) was used. Statistical significance was calculated using unpaired two-tailed Student’s t-test. b, Blood analysis at the end of the experiment show that ouabain treatment does not change immune composition. Blood from young (n = 8) and old mice, either treated with vehicle (n = 6) or ouabain (n = 8) was used. Statistical significance was calculated using unpaired two-tailed Student’s t-test. c–d, Representative IHC images (c) and quantification (d) of the indicated immune cell markers in the liver of young (n = 6) and old mice, either treated with vehicle (n = 6) or ouabain (n = 8). Scale bar: 100µm. Statistical significance was calculated using one-way ANOVA with Tukey’s post hoc comparison. Data represent mean ± s.e.m.; n represents number of mice; ns, not significant.
