Extended Data Fig. 8: Effect of hydrogen sulfide early in life on colitis susceptibility.
From: Excess calorie intake early in life increases susceptibility to colitis in adulthood
Weight of (a) total body, (b) epididymal fat, (c) retroperitoneal fat, (d) liver, (e) intestinal permeability and (f) colonic expression of mRNA for cytokines and MLCK and epithelial tight junction proteins at 4 weeks of age in germfree mice grown in small litter (SL) and exposed to slow release of H2S (GYY4137) and/or heat-killed microbiota (M), or to vehicle control during 2 to 4 weeks of age. (g) Percentage of body loss, (h) disease activity index, (i) colonic length and (j) colonic transcript profile after colitis induction in adult germfree mice exposed to a slow-release H2S donor (GYY4137) and/or heat-killed microbiota (M) or to vehicle control, and grown in SL, during 2 to 4 weeks of age. Post hoc Tukey-adjusted tests following significant (P<0.05, after correction) Analyses of Deviance (mixed-effect models), all ****P<0.0001; d (***P=0.0005); f (Il6 *P=0.02; Il22 *P=0.03; Tjp1 *P=0.04; Tjp2 **P=0.004); g (*P=0.03, ***P=0.0005); h (*P=0.04); i (***P=0.0005); j (***P=0.0008). ns= not significant. For SL+Vehicle (a-e (n=8; litters=7), f (n=7; litters=6), g-j (n=6; litters=6)); SL+GYY4137 (a-e (n=8; litters=6), f (n=5; litters=4), g-j (n=6; litters=6)); SL+M (a-e (n=8; litters=7), f (n=6; litters=5), g-j (n=6; litters=6)); SL+M+GYY4137 (a-e (n=10; litters=9), f (n=8; litters=7), g-j (n=8; litters=8)); No colitis (g-j (n=7; litters=7)). Data were pooled from at least two independent experiments. Each dot represents 1 offspring mouse. Dots of same color and symbol represent mouse from same litter. Data are shown as mean±s.e.m.
