Extended Data Fig. 5: Generation and baseline characterization of cardiac-specific ABHD5 transgenic mice (TG).
From: The lipid-droplet-associated protein ABHD5 protects the heart through proteolysis of HDAC4
a, Microinjected fragment of an expression plasmid containing hABHD5 cDNA flanked by α-MHC promoter and human growth hormone (hGH) poly-A (+) signal. b, Cardiac overexpression of ABHD5 in mice of 4 different transgenic lines as compared to wild-type littermates (WT) from the same lines was evaluated by immunoblotting analysis using an anti-ABHD5 antibody. c–e, Baseline characterization of transgenic mice. c, Representative echocardiography of a mouse from transgenic line #1 as compared to a WT littermate. The data in b are representative of 4 different mouse lines and the data in c are representative of 4 mice from line # 1 as well as 7 (WT) and 10 (TG) mice from line #9. d,e, Quantification of ejection fraction (EF), heart weight/body weight ratio (HW/BW), heart weight/tibia length ratio (HW/TL) in WT littermates (n = 4) and transgenic mice from line #1 (n = 4) (d) and in WT littermates (n = 7) and transgenic mice from line #9 (n = 10, TG) (e). d,e, Statistical analysis: Values are presented as mean ± s.e.m.; by unpaired two tailed t-test, P < 0.05 considered as significant. n represents mice per indicated group.
