Extended Data Fig. 4: ABHD5 inhibits MEF2 and cardiomyocyte hypertrophy.
From: The lipid-droplet-associated protein ABHD5 protects the heart through proteolysis of HDAC4
a, NRVMs were transduced with the Ad3×MEF2C-Luc reporter. Ad.FLAG–HDAC4, Ad.ABHD5 plus GFP on a bicistronic promoter and Ad.GFP (10 MOI) were then coexpressed as indicated, and NRVMs were stimulated for 24 h with ET-1 or FCS; n = 5 (n represents independent samples) and values are presented as mean ± s.e.m. b, Right panel: Fluorescence microscopy of NRVMs expressing Ad.ABHD5 plus GFP on a bicistronic promoter or Ad.GFP (10 MOI each). NRVM hypertrophy was induced with 100 nM ET-1 or 10% FCS for 24 h. Shown are representative images of α-actinin staining (red) of NRVMs and GFP fluorescence to confirm adenoviral expression. Images are representative of at least three independent experiments. Scale bar, 50 μm. Right panel, Quantification of NRVM cross-sectional areas (CSA). Statistical analysis: Values are presented as mean ± s.e.m., n = 4 (n represents independent samples); by one-way ANOVA, P < 0.05 considered as significant.
