Extended Data Fig. 2: ECM stiffness directs creatine metabolism in PDAC cells. | Nature Metabolism

Extended Data Fig. 2: ECM stiffness directs creatine metabolism in PDAC cells.

From: The creatine–phosphagen system is mechanoresponsive in pancreatic adenocarcinoma and fuels invasion and metastasis

Extended Data Fig. 2: ECM stiffness directs creatine metabolism in PDAC cells.The alternative text for this image may have been generated using AI.

(a) Schematic representation of the phosphocreatine circuit. Red indicates metabolite enrichment on soft (0.7kPa) ECM, while blue indicates enrichment on stiff (glass) ECM. (b) Urea cycle and creatine biosynthesis metabolic intermediates of KPC cells cultured on fibronectin-coated 0.7–38 kPa hydrogels and glass coverslips as indicated. Values are mean ± SD from 3 biological replicates within the same day. Statistical significance assessed by one-way ANOVA. (c) Urea cycle and creatine biosynthesis metabolic intermediates of PANC-1 cells cultured as indicated. Values are mean ± SD from 3 biological replicates within the same day. Statistical significance assessed by two-tailed unpaired t-test with Welch’s correction. (d) Arginine-derived labelled carbon and nitrogen incorporation in urea cycle and creatine biosynthesis metabolites of KPC cells cultured as indicated and supplemented with L-arginine-13C615N4 for 1, 3 and 6 hours. Values are mean ± SD from 3 biological replicates within the same day.

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