Extended Data Fig. 5: Senescent cell burden is causally linked to increased macrophage CD38 expression.
From: Senescent cells promote tissue NAD+ decline during ageing via the activation of CD38+ macrophages
a, mRNA levels of Il-1α, Cxcl1, and IL-10 in eWAT from 6 month-old WT male mice i.p. injected with Doxo or PBS. (PBS n=8 mice/group, Doxo n=7 mice/group) b, Quantification of CD38-low resident macrophages, and CD38-low non-resident macrophages from eWAT of 6 month-old WT male mice injected with Doxo or PBS. (PBS n=8 mice/group, Doxo n=8 mice/group). c, CD38 mRNA levels in WT and Cd38 KO BMDMs co-cultured (10:1) with non-senescent control mouse dermal fibroblasts (CTRL-MDF) or irradiated senescent MDF (Sen(IR)-MDF) for 24 hours. (n=4 independent biological experiments per condition) d, mRNA levels of Cd38 in WT BMDMs treated with the indicated DAMPs for 16 hours. (n=3 independent biological experiments per condition) e, mRNA levels of inflammatory genes in CTRL-MDF and Sen(IR)-MDF. (n=4 independent biological experiments per condition) f, mRNA levels of Cd38 in BMDMs treated with the indicated concentrations (ng/ml) of recombinant mouse cytokines for 24 hours. (n=3 independent biological experiments per condition) g, Heatmap of significantly upregulated proteins identified by mass spectrometry in conditioned media from CTRL-MDF and Sen(IR)-MDF. (n=4–6 independent biological experiments per condition). For in vivo experiments, data from individual mice are shown. Data show the mean ± SEM. (n= at least 3 independent experiments). Statistical significance indicated as *P<0.05, **P<0.01, and ***P<0.001; two-sided Student’s t-test.
