Extended Data Fig. 6: Generation and validation of EC-lineage tracing mice and EC-specific deletion of Il1r1. | Nature Metabolism

Extended Data Fig. 6: Generation and validation of EC-lineage tracing mice and EC-specific deletion of Il1r1.

From: Multiple cell types contribute to the atherosclerotic lesion fibrous cap by PDGFRβ and bioenergetic mechanisms

Extended Data Fig. 6: Generation and validation of EC-lineage tracing mice and EC-specific deletion of Il1r1.

a, Cdh5-CreERT2/ROSA-STOPFL/FL-eYFP/Apoe−/− (Cdh5-CreERT2) mice were generated to lineage trace EC and their progeny within the BCA. Mice were then crossed to an Il1r1-Flox mouse to generate Il1r1EC−fl/fl and −WT/WT animals. Heterozygote animals were bred (Il1r1Fl/WT; Cdh5-CreERT2/ROSA-STOPFL/FL-eYFP/Apoe−/−) to generate littermate FL/FL and WT/WT controls, which were used in all experiments. b, Image denoting the endothelial monolayer with Cdh5-eYFP+ staining only in this layer. c, Validation of genetic deletion of Il1r1 in organs using isolated DNA. Lanes A, C, E contain the FL and WT genotyping reactions while lanes B, D, F contain the excision reaction. Representative images of Cdh5-eYFP+ staining in (d) Liver, (e) Lung, and (f) Aorta. g, Gating strategy for isolation of Cdh5-eYFP+ endothelial cells. h, Genotyping and recombination analysis of Cdh5-eYFP+ cells sorted from Il1r1EC-WT/WT (lanes A, B) and Il1r1EC-FL/FL (lanes C, D) mice. Lanes A, C contain FL and WT genotyping reaction while B, D contain the excision reaction. Scale bar: 100 μm (d,e,f).

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