Extended Data Fig. 7: Lethal radiation and BMT significantly increases the percentage of EC in the lesion and fibrous cap. | Nature Metabolism

Extended Data Fig. 7: Lethal radiation and BMT significantly increases the percentage of EC in the lesion and fibrous cap.

From: Multiple cell types contribute to the atherosclerotic lesion fibrous cap by PDGFRβ and bioenergetic mechanisms

Extended Data Fig. 7: Lethal radiation and BMT significantly increases the percentage of EC in the lesion and fibrous cap.

a, Representative images of BCA lesions in Cdh5-CreERT2 mice fed 18 weeks of WD with or without 1200cGy of radiation, which is known to ablate SMC accumulation within the lesion and fibrous cap. b, Quantification of Cdh5-eYFP+ cells in the fibrous cap showed significantly increased EC-derived cells after radiation. ***p-values < 0.0001. c, The increased percentage of EC-derived cells within the fibrous cap was not associated with changes in overall ACTA2+ cells within the fibrous cap. d, Zoom in of highlighted area in (a) showing EC-derived cells express ACTA2. Single cell breakdown and quantification of Cdh5-eYFP+ ACTA2+ cells per total Cdh5-eYFP+ (e) or per total ACTA2+ cells, **p-value = 0.0023, (f) within the fibrous cap, ***p-values < 0.0001. g, Representative image of bone marrow cells (BMC) expressing ACTA2 within the fibrous cap of Cdh5-CreERT2 mice after radiation. h, Quantification and comparison of MMT by co-incident staining of ACTA2 and marker protein staining for LGALS3 or for the BMC lineage tag, tdTomato. Scale bar: 100 μm (whole lesion) or 50 μm (zoom). Graphs were analyzed using two-tailed Mann-Whitney U test, error bars represent mean ± SEM.

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