Extended Data Fig. 4: Effect of pertussis toxin on PTH secretion from PTGs.
PTGs (2 per group) from wild-type C57/B6 were sequentially incubated with increasing [Ca2+]e from 0.5 to 2.0 mM (1 hr for each concentration) in the presence of vehicle (0.1% DMSO) or baclofen (Bac, 300 µM) with or without preincubation with pertussis toxin (PTx, 100 µg/ml, 3 hrs). Mean ± s.e.m. of n pairs of PTGs from n mice as indicated. P values vs Vehicle controls were assessed by 2-way ANOVA with Sidak’s test.
