Extended Data Fig. 4: The exercise-induced improvement in old MuSC activation is transferable through serum. | Nature Metabolism

Extended Data Fig. 4: The exercise-induced improvement in old MuSC activation is transferable through serum.

From: Exercise rejuvenates quiescent skeletal muscle stem cells in old mice through restoration of Cyclin D1

Extended Data Fig. 4: The exercise-induced improvement in old MuSC activation is transferable through serum.

a, Old recipient mice that had never exercised received three consecutive daily tail-vein injections with serum collected from old non-exercising or exercising mice. MuSCs were isolated from recipient mice one day after the last injection. b, FACS-isolated MuSCs were cultured continuously in EdU to assess S-phase progression (n=8 recipient mice for O(-Ex), comprising 4, 3, and 1 recipients for three different serum pools, and n=6 recipient mice for O(+Ex), comprising 3, 2, and 1 recipients for three different serum pools). Data are summarized with mean + s.e.m. *P<0.05; two-tailed Mann-Whitney U-test in b.

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