Extended Data Fig. 8: Liver depletion of MRG15 leads to reduced expression of lipid and cholesterol synthesis genes, and inflammatory genes in HFD animals.
From: MRG15 orchestrates rhythmic epigenomic remodelling and controls hepatic lipid metabolism
Tissues were collected from animals under HFD after virus injection for 14 weeks for RNA-seq analysis. a, b, c, Representative genes downregulated in CRISPR-Mrg15 livers involved in triglyceride synthesis (a), cholesterol synthesis (b) and inflammatory response (c). Values are indicated as log 2 [Fold change (CRISPR-Mrg15/Control)] in a, b and c. Genes that are direct targets of MRG15 as revealed in ChIP–seq analysis in a and b are labelled with an asterisk at upper right. d, Gene enrichment analysis of downregulated genes in CRISPR-Mrg15 livers identified by RNA-seq (622 genes using a cut-off of P < 0.05). Associated P values were determined according to the analysis in the GO database (Fisher’s exact P value). e, Relative mRNA expression of representative lipid genes in liver in control and Mrg15-sgRNA treated animals under HFD. n = 9 biologically independent samples. P values are shown for indicated comparisons by the two-tailed Mann–Whitney U tests. Values are mean ± s.e.m.
