Extended Data Fig. 7: The impact of C12orf49 loss on the cleavage of MBTPS1 targets.
a, Immunoblot analysis of OS9 in the C12orf49 immunoprecipitates of the HEK293T cell line expressing the indicated cDNAs. The experiment was repeated independently twice with similar results. b, Immunoblot analysis of cleavage of other site-1 protease targets, GNPTAB, CREB3L2 and CREB4 at 24 h following transfection in the C12orf49-knockout and addback HEK293T cells. Actin was used as loading control. The experiment was repeated independently twice with similar results. c, Localization of SCAP-GFP in c12orf49 null HEK293T cells expressing control or C12orf49 cDNA under lipoprotein depletion in the presence or absence of sterols (Scale bar, 8 µm). The experiment was repeated independently twice with similar results.
