Extended Data Fig. 5: Role of C12orf49 in sterol synthesis and SREBP-mediated transcription.
a, (top left) Percentage of Bunyamwera virus-positive cells at 72 h post-infection (MOI=0.1IU/Ml) in indicated knockout and addback HEK293T cells (mean ± SD, n=3 biologically independent samples). Statistical significance was determined by two-tailed unpaired t-test. (top right) Viral titer measured by TCID50 assays on BHK-21 cells with the harvested supernatant from the Bunyamwera virus infected HEK293T cells of C12orf49 knockouts and addbacks. (mean ± SD, n=3 biologically independent samples) Statistical significance was determined by two-tailed unpaired t-test. (bottom) Growth of the viral titers at different time points in the knockout and addback cells. b, Fold change in mRNA levels (log2) of SREBP target genes in indicated Jurkat cell lines following 8h growth under lipoprotein depletion in the presence and absence of sterols (mean ± SD, n=3). c, Relative luminescence activity (Luciferase/Renilla) in the indicated HEK293 cell lines following transfection with firefly luciferase under SRE promoter and Renilla luciferase for normalization of transfection following 24h growth under lipoprotein depletion in the presence and absence of sterols (mean ± SD, n=3 biologically independent samples). Statistical significance was determined by two-tailed unpaired t-test.
