Extended Data Fig. 5: Itaconate treatment increases succinate accumulation and inhibits SDH activity.
a, Intracellular itaconate versus intracellular succinate accumulation in BMDMs after 12h treatment with the indicated compounds (n = 8 cultures). b, Intracellular succinate levels in BMDMs after 12h treatment with DI (n = 8 cultures) c, SDH activity in the presence of the indicated compounds (n = 5 cultures except for 5 NaITA, 7.5 NaITA n = 3 cultures; 10 NaITA n = 2 cultures; statistics not shown for n < 3). All compounds were neutralized with sodium hydroxide before adding to the assay. *P < 0.05, ***P < 0.001, ****P < 0.0001, P values calculated using one way ANOVA. Exact P values listed in Supplementary Information in Source Data files. d, Time course of itaconate and succinate accumulation after LPS stimulation in WT BMDMs (n = 6 cultures). Treatment concentrations for all experiments listed in mM. All bar plots representative of mean + s.e.m., except where n < 3 only the mean is depicted. AU = arbitrary units based on mass spectrometry peak area and is not directly comparable between experiments.
