Extended Data Fig. 5: Regulation of LDL uptake and LDLR expression by C12orf49.

a, Bar plots showing the results of a low density lipoprotein (LDL) uptake assay across the indicated HAP1 cell lines using pHrodo-LDL probe. All data are represented as means ± standard deviation; n = two (SREBF1, SREBF2) or three (WT, FASN, LDLR, C12orf49, WT + C12orf49-V5, C12orf49 + C12orf49-V5) biologically independent experiments; one-way ANOVA. b, Bar plots showing the results of a transferin uptake assay across the indicated HAP1 cell lines using pHrodo-Transferin probe. All data are represented as means ± standard deviation; n = two (SREBF1, SREBF2) or three (WT, FASN, LDLR, C12orf49, WT + C12orf49-V5, C12orf49 + C12orf49-V5) biologically independent experiments; one-way ANOVA. c, Bar plots showing the results of a low density lipoprotein (LDL) uptake assay across the indicated in lipoprotein-deprived HAP1 cell lines using Dil-LDL probe. All data are represented as means ± standard deviation; n = two biologically independent experiments. d, Pathway enrichment analysis of BioID preys captured with N-terminal (top panel) or C-terminal (bottom panel) miniTurbo-tagged C12orf49 under normal growth conditions using the GO molecular function, biological process, cellular compartments, KEGG and Reactome standards. Terms for significantly enriched gene sets (p < 0.05, maximum term size 105) are indicated and bars depict mean percentage overlap with the indicated term. Numbers next to each bar indicate the mean overlap and term sizes, respectively. The greyscale color legend for p-values is indicated on the right. P-values were calculated using gProfileR⁶⁹. e, Barplots depicting the number of proteins localizing to indicated cellular compartments for preys captured with N-terminal (grey) or C-terminal (black) miniTurbo-tagged C12orf49 in 293 cells under serum-starvation.