Extended Data Fig. 7: Gating strategy for flow cytometry experiments. | Nature Metabolism

Extended Data Fig. 7: Gating strategy for flow cytometry experiments.

From: Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism

Extended Data Fig. 7

Gating strategies for flow cytometry experiments for a, co-culture assays and b, LDL/Transferrin uptake assays. In all cases the following steps were taken: 1. Forward scatter area (FSC-A) vs. side scatter area (SSC-A) were used to separate cell events from debris. 2. Forward scatter height (FSC-H) vs. forward scatter width (FSC-W) was used separate single cells from aggregates. 3. Forward scatter area (FSC-A) vs. viability stain (7AAD/B695-40 or Zombie NIR/R780-60) was utilized to gate live cells. For co-culture assays, gating scheme for separating GFP/B530-30 vs RFP/YG610-20 positive cells including steps 1-3 are shown in panel (a). For Dil-LDL/YG585-15 quantification, marker-positive live cells were quantified relative to unstained controls following steps 1-3 as displayed panel (b).

Back to article page