Extended Data Fig. 2: Quality control of genetic interaction screens for fatty acid synthesis-related query genes. | Nature Metabolism

Extended Data Fig. 2: Quality control of genetic interaction screens for fatty acid synthesis-related query genes.

From: Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism

Extended Data Fig. 2

a, Precision-recall curves distinguishing the reference core essential gene set (CEG2) defined in Hart et al., 201719 and a non-essential gene set in CRISPR screens in five HAP1 knockout query cell lines (LDLR, C12orf49, SREBF2, ACACA, SREBF1-KO). b, Fitness effect (LFC) distributions for reference core essential (CEG2) and non-essential gene sets defined in Hart et al., 201719 across CRISPR screens in five HAP1 KO cell lines (LDLR, C12orf49, SREBF2, ACACA, SREBF1). c, Bar plot of enrichment of co-annotation as defined by the Human Functional Network, Gene Ontology Bioprocesses (BP), HumanCyc or and aggregation of pathway standards (including REACTOME, KEGG or BIOCARTA) for genetic interactions identified across all six query genome-wide screens (FASN, LDLR, C12orf49, SREBF2, ACACA, SREBF1). Enrichment was tested using a hypergeometric test. See methods for details of analysis.

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