Extended Data Fig. 4: In vivo and ex vivo characterization of parameters reflecting PH, right heart structure and function, and histology of the vasculature in WT and Noxo1−/− mice with and without smoke exposure.
From: NADPH oxidase subunit NOXO1 is a target for emphysema treatment in COPD
a, Transthoracic echocardiography after 8 months of smoke exposure: i, Representative echocardiographic images, showing the short axis view of the right ventricle (RV), the four-chamber view demonstrating dilation of the RV (RV internal diameter: RVID), the pulmonary flow (pulmonary artery acceleration time: PAAT) which is inversely correlated to the RV systolic pressure (RVSP), and the tricuspid annular plane systolic excursion (TAPSE). ii–v, Quantitative analysis of PAAT (ii, n = 11 mice each for WT and n = 13 mice each for Noxo1−/−), right ventricular wall thickness (iii, RVWT, n = 10 mice for WT and n = 13 mice each for Noxo1−/− groups), RVID (iv, n = 10 mice each for WT and n = 13 mice each for Noxo1−/−) and TAPSE (v, n = 9 mice for 8 m RA WT, n = 10 mice for 8 m SE WT, n = 13 mice for 8 m RA Noxo1−/−, n = 11 mice for 8 m SE Noxo1−/− group). b–d, Parameters of PH after 3 months of smoke exposure. b, RVSP (n = 12 mice each, except n = 10 for 3 m SE Noxo1−/−) and c, Ratio of RV mass to left ventricular + septum mass (RV/(LV + septum). No changes in left ventricular mass were detectable among the different groups (n = 12 mice each, except n = 11 for 3 m SE Noxo1−/−). d, Vascular remodelling by quantification of muscularization degrees of small vessels, given as average of all muscularization, in lung sections co-stained against α-smooth muscle actin and von Willebrand factor (n = 4 lungs each). e, Representative histologic images from lung sections co-stained against α-smooth muscle actin and von Willebrand factor, demonstrating vascular remodelling after 8 months of smoke exposure. Statistical analysis: a, c: 2 × 2 factorial models on log-transformed data; p-values are from 2-sided t-tests. b: p-values are from Tukey HSD tests using the log-transformed data. d: 2 × 2 factorial models on logit-transformed data; p-values are from 2-sided t-tests. Data are represented as single value and mean ± s.e.m.
