Extended Data Fig. 1: Tissue screens for CCL11 and CCL2 protein expression in young and aged mice.
a, Comparison of CCL11 and CCL2 protein expression in muscle, spleen, brain, kidney, thymus, liver, lung, skin, mesenteric lymph nodes (MLN), colon, subcutaneous WAT (scWAT) and epidydimal WAT (eWAT) of young (Y, 2-3 months) and aged mice (A, 18-20 months) as assessed by western blot. Tissues of three biologically independent animals were pooled. HSP90 served as loading control. Quantification of b, CCL11 and c, CCL2 protein levels normalized to HSP90 in indicated tissues of young (Y, 2-3 months) and aged mice (A, 18-20 months) by ImageJ. One out of two independently performed experiments is shown. d, Comparison of CCL11 and CCL2 mRNA expression levels in indicated tissues of aged mice (18-20 months) as assessed by qPCR (n=4). e, CCL2 and CCL11 protein levels were assessed by western blot (n=4). One out of 3 independently performed experiments is shown. f, Quantification of CCL11 and CCL2 protein levels normalized to HSP90 in indicated tissues of young (Y, 2-3 months, n=4) and aged mice (A, 18-20 months, n=4). HSP90 served as loading control. Protein levels from total eWAT was calculated. Statistical significance was calculated by one-way ANOVA followed by two-tailed post-hoc Dunnett’s multiple comparison test against eWAT (d) or by unpaired two-tailed Student’s t test between young and aged samples (f). Data are shown as individual data points with mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. ns, not significant. Uncropped western blots are provided in the Source Data File.
