Extended Data Fig. 2: Gating strategies for human and mouse ATEs and age-related adipose tissue hypertrophy.

a, Gating strategy for human omental adipose tissue eosinophils. b, Representative images of H&E stained human omental adipose tissue from young and aged donors of indicated age. Scale bar: 100 μm. c, Quantification of adipocyte size in H&E stained sections from human omental adipose tissue (n=17 biologically independent human donors) by ImageJ. d, Representative gating strategy for ATE (F4/80^int, SiglecF⁺) and ATM (F4/80⁺, SiglecF^-) in mouse visceral adipose tissue of young (3 months) and aged mice (20 months). e, Absolute eosinophil and macrophage numbers per gram eWAT of young (3 months, n=10) and aged mice (20 months, n=10). This experiment was done once. f, Representative photographs of H&E stained histological eWAT sections of indicated treatment groups. g, Quantification of adipocyte hypertrophy in young (n=20), Aged-PBS (n=19), Aged-yEOS (n=22) and Aged-yEOS^IL-4-/- (n=20) biologically independent mice by ImageJ. Statistical significance was calculated by unpaired two-tailed Student’s t test (e), the Pearson correlation coefficient between adipocyte size and age (c) or by one-way ANOVA followed by two-tailed post-hoc Dunnett’s multiple comparison test against the aged-PBS treated group (g). Data are shown as individual data points with mean bars ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. ns, not significant.