Extended Data Fig. 3: Recruitment of sort-purified GFP⁺ eosinophils to WAT of aged mice.

Aged mice (18 months) were adoptively transferred with sort-purified GFP⁺ eosinophils derived from IL-5 transgenic mice on two subsequent days. The following day eosinophil recruitment into different tissues was assessed by flow cytometry. a, Representative flow plots of transferred GFP⁺ and endogenous GFP^– tissue eosinophils in indicated tissues. b, Frequencies of transferred GFP⁺ and endogenous GFP^– tissue eosinophils in indicated tissues. c, Siglec-F surface expression on adipose tissue eosinophils from Young, Aged-PBS and Aged-yEOS -treated mice as assessed by flow cytometry. d, Representative histograms of Siclec-F expression on ATEs of indicated groups. e, Quantification of Siglec-F surface expression (MFI) on adipose tissue eosinophils of Young (n=5), Aged-PBS (n=5) and Aged-yEOS (n=4) mice. The experiment was done once. Statistical significance was calculated by one-way ANOVA followed by two-tailed post-hoc Dunnett’s multiple comparison test against the young group. ns, not significant. Data are representative of n=4 per group and are shown as mean ± SEM.