Extended Data Fig. 10: Eosinophils adopt a senescent-like inflammatory phenotype with age.

a, Heat map representing Ct values of p21, Vegfa, Il1b, Il6 and Tnfa in sort-purified, blood-derived eosinophils from aged WT (20 months), young WT (3 months) or young IL5 transgenic mice (3 months) as assessed by targeted fluidigm qPCR array. b, Relative expression levels of Tnfa, Il1b, Il6, p21 and Vegfa in sort-purified, blood-derived eosinophils from aged WT (20 months), young WT (3 months) or young IL5 transgenic mice (3 months) as assessed by fluidigm qPCR array. Eosinophils from 3 animals were pooled for each measurement (n=3 per group). The experiment was done once. c, Relative expression levels of TNFa, IL1b, IL6, p21 and VEGFA in human blood derived eosinophils from young (average age=34, n=8) and aged (average age=64, n=7) donors. Data in (b and c) are shown as individual data points with mean ± SEM and statistical significance was calculated by one-way ANOVA followed by two-tailed post-hoc Dunnett’s multiple comparison test against the young group (b) and two-tailed students t-test (c). *p < 0.05, **p < 0.01, ***p < 0.001. ns, not significant. ND, not detectable.