Extended Data Fig. 4: AMPKα1 expression in NF200, PARV, CGRP and IB4 positive DRG neurons.

a, Representative fluorescence images of immunostaining for AMPKα1 and parvalbumin (PARV) or CGRP, or IB4+ in DRG neurons. n = 3. Scale bar, 100 μm. b, Representative fluorescence images of immunostaining for AMPKα1 and parvalbumin (PARV) in DRG neurons following Sham and SNA. n = 3. Scale bar, 100 μm. c, Percentage of NF200, PARV, CGRP or IB4 positive neurons expressing AMPKα1. d, Quantification of immunostaining for AMPKα1 expression level of (b). n = 3 mice each group. Each mouse represents an independent replicate. The relative AMPKα1 expression level was quantified after normalization to the background (secondary antibody only). Values represent means ± SEM. Two-tailed unpaired Welch’s t-test.