Extended Data Fig. 5: AMPKα1 expression in DRG neurons following AMPKα1 conditional deletion or overexpression. | Nature Metabolism

Extended Data Fig. 5: AMPKα1 expression in DRG neurons following AMPKα1 conditional deletion or overexpression.

From: AMPK controls the axonal regenerative ability of dorsal root ganglia sensory neurons after spinal cord injury

Extended Data Fig. 5

a, Representative fluorescence images of immunostaining for GFP; AMPKα1 and DAPI in cultured DRG cells dissected from AMPKα1 floxed mice 48 h after transfection with AAV-GFP or AAV-Cre-GFP. n = 3. Scale bar, 100 μm. b, Representative fluorescence images of immunostaining for GFP; AMPKα1 and DAPI in cultured DRG cells after electroporation with GFP plasmid or AMPKα1 plasmid at 48 h. Arrows show non-pycnotic DAPI positive nuclei. Scale bar, 50 μm. c, Quantification of the percentage of cells with non-pycnotic nuclei (b). n = 3 independent experiments. Values represent means ± SEM. Two-tailed unpaired Welch’s t-test.

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