Extended Data Fig. 8: GFP and dextran co-localization in DRG neurons.
a, Representative images of DRG sections from AAV-Cre-GFP sciatic nerve injected mice co-immunostained with antibodies against GFP and Dextran. Scale bar, 50 μm. b, c, Quantification of the percentage of GFP+ and Dextran+ / GFP+ cells following AAV-GFP or AAV-Cre-GFP. AAV-GFP, n = 13 mice; AAV-Cre-GFP, n = 10 mice. Percentage of GFP positive cells was calculated as the ratio of GFP+ versus TUJ1+ cells; percentage of Dextran+/GFP+ was calculated as the ratio of Dextran+/GFP+ versus TUJ1+ cells. Values represent means ± SEM. Two-tailed unpaired Welch’s t-test. d, Longitudinal spinal cord section 5 weeks after SCI showing axonal labelling across the injured dorsal columns following deletion of AMPKα1. Dorsal column axons are labelled by sciatic nerve injected Dextran. Asterisk indicates the lesion epicentre. D; dorsal; V; ventral, C; caudal; R; rostral. Scale bar; 250 μm. Similar results were found in eight AMPKα1 conditionally deleted mice. The quantification source data is provided in statistical source data, Fig. 8b.
