Extended Data Fig. 1: The plasma lipidome is altered by Western diet.
From: Diet-dependent regulation of TGFβ impairs reparative innate immune responses after demyelination
a, PCA analysis of CD and WD plasma samples. b, Mol% of the sample occupied by the different lipid classes in CD- and WD-fed mice (mean ± SD, two-tailed Welch’s t-test). c,e, Mol% of each sample with lipids containing a certain number of double bonds (c) or a certain acyl chain length (e) (mean ± SD, two-tailed Welch’s t-test). d, Heatmap showing the significantly (p value ≤ 0.01, fold ≥2.5) changed lipid species in plasma from CD- and WD-fed mice. Scale indicated on top of the heatmap. Increased values are indicated in orange, while decreased values are in blue. f, Quantification of the glucose level in blood at baseline (0 minutes) and 15, 30, 60 and 120 minutes after intraperitoneal glucose administration. (mean ± SD, two-tailed Welch’s t-test). g, PCA analysis of plasma from young (3-months old) versus old (12-months old) mice. h, Mol% of the sample occupied by the different lipid classes in plasma from young and old mice (mean ± SD, two-tailed Welch’s t-test). i,k, Mol% of each sample with lipids containing a certain number of double bonds (i) or a certain acyl chain length (k) in plasma from young and old mice (mean ± SD, two-tailed Welch’s t-test). j, Heatmap showing the significantly (p value ≤ 0.05, fold ≥1.3) changed lipid species in plasma from young and old mice. Scale indicated on top of the heatmap. Increased values are indicated in orange, while decreased values are in blue. P-values below 0.1 and n numbers are indicated in the figures. PCA: principal component analysis, CD: control diet, WD: Western diet, CE: cholesterol esters, Chol: cholesterol, DAG: diacylglycerol, LPC: lysophosphatidylcholine, PC: phosphatidylcholine, TAG: triacylglycerides, PI: Phosphatodylinositol. In d and j, the lipids are numbered according to the carbon length; number of double bonds; and number of hydroxyl groups (for example TAG 52;3;0).
