Extended Data Fig. 4: (Related to Fig. 4): Phenotype in the liver of Hmgcs2 KO. | Nature Metabolism

Extended Data Fig. 4: (Related to Fig. 4): Phenotype in the liver of Hmgcs2 KO.

From: Murine neonatal ketogenesis preserves mitochondrial energetics by preventing protein hyperacetylation

Extended Data Fig. 4: (Related to Fig. 4): Phenotype in the liver of Hmgcs2 KO.The alternative text for this image may have been generated using AI.

a, Section electron microscopy of postnatal day 3 (P3) hepatocytes. Red arrows represent peroxisomes. Scale bar = 0.5 µm. b, Protein content is represented as weight percent (%). WT; Hmgcs2 WT, n = 7, 13.1 ± 0.82%, KO; Hmgcs2 KO, n = 9, 9.10 ± 0.59%. c, Gas chromatography-mass spectrometry (GC-MS) based concentrations of citrate, alpha-ketoglutarate, fumarate, and malate, adjusted by each sample weight. Hmgcs2 WT (n = 6), Hmgcs2 KO (n = 8). d, GC-MS based concentrations of citrate, alpha-ketoglutarate, fumarate, and malate, adjusted by each protein content. Hmgcs2 WT (n = 6), Hmgcs2 KO (n = 8). e, Basal respiration, ATP production, and maximal respiration of primary hepatocytes from P3 Hmgcs2 WT and KO neonates (Related to Fig. 4g). f, Gating strategy of flow cytometry (Related to Fig. 4h). All results are expressed as means ± standard deviation (SD). Welch’s two-sided t-tests were performed (c-e).

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