Extended Data Fig. 5: (Related to Fig. 5): Mitochondrial protein acetylation in Hmgcs2 KO livers.
a, Heatmap for iMPAQT analysis (WT = 3, KO = 2). Green characters represent mitochondrial proteins. b, Protein fold changes between Hmgcs2 KO and WT mice (Related to Fig. 5a). c, Immunoprecipitation assays on P7 livers: IP, anti-acetylated lysine (AcK); IB, anti-LCAD. Red arrowheads indicate LCAD protein. LCAD, long-chain acyl-CoA dehydrogenase. d, Immunoprecipitation assays on postnatal day 7 (P7) livers: IP, anti-LCAD; IB, anti-acetylated lysine (left), anti-LCAD (right). Red arrowheads indicate LCAD protein. 800 µg purified mitochondrial proteins were used for immunoprecipitation assays. e, Serial changes in protein acetylation in the liver. Whole protein (10 µg) from WT livers (E18.5, P3, P7, P21, and Adult) was blotted using the anti-acetylated lysine antibody. (AcK; acetylated lysine). f, Western blot analysis for GCN5L1, UQCRFS1, and HMGCS2 between P7 Hmgcs2 wild type and knockout livers (n = 4 each). Welch’s two-sided t-test was performed. g, Expressions of Acly, Acss2, and Slc25a1 mRNA, adjusted by Rplp1 in P7 neonatal livers. (Hmgcs2 WT, n = 4, KO, n = 4). Welch’s two-sided t-test was performed. Results are expressed as means ± standard deviation (SD). h, Western blot analysis for AcK and total protein (TP) between RosaCAG-Sirt3 and wild-type adult heart (n = 4 each). Welch’s two-sided t-test was performed.
