Extended Data Fig. 6: Islet preparations contain only few non-endocrine cells.

(a) Infected pancreatic islets of donor 3 were stained for endothelial marker CD31 (green) and insulin (red) and compared to primary human umbilical vein endothelial cells (HUVEC) cells as positive control. Cell nuclei were visualized by DAPI (blue). (b) Pancreatic islets were stained for pancreatic ductal marker cytokeratin 19 (CK19, green) and cell nuclei were visualized by DAPI (blue). (c) Infected pancreatic islets were co-stained for pancreatic acinar cell marker chymotrypsin (red) and viral N protein (green). Representative laser-scanning confocal images selected from at least 4 pancreatic islets are shown, scale bars depict 10 µm (a-c). (d) Infected pancreatic islets of donor 3 (day 5) were co-stained for pancreatic β-cell marker NKX6.1 (green) and viral SARS-CoV-2 N protein (red). Cell nuclei were stained with DAPI (blue). Representative fluorescence images selected from 11 pancreatic islets are shown, scale bars depict 10 µm. (e) Fraction of NKX6.1 and N protein double-positive cells were quantified in infected islet clusters of donor 3. Data are presented as mean values ± s.e.m. from 11 individual islet clusters positive for N protein.