Extended Data Fig. 3: MTKO mice are susceptible to obesity and have reduced energy expenditure on a high-fat diet.
From: Insulin-stimulated endoproteolytic TUG cleavage links energy expenditure with glucose uptake
a, Mice were fed a high-fat diet (HFD) beginning at 15 weeks of age, and the percentage weight gain from baseline is plotted. N=10 WT and 12 MTKO mice. b, After the HFD, HOMA-IR was calculated from paired measurements of plasma glucose and insulin. N=7 WT and 10 MTKO mice. c, Immunoblots were done as indicated on hindlimb muscles from mice fed a HFD for 3 weeks. d–f, Body weights and composition of 14-week old MTKO and WT mice fed a HFD for 3 weeks prior to measurements done in metabolic cages. N=7 in each group. g–q, HFD-fed 14-week old MTKO and WT mice were housed in metabolic cages and the indicated parameters were measured. N=7 in each group (g–l, p, q); N=7 WT and 6 MTKO mice (m–o). RER, respiratory exchange ratio; LBM, lean body mass. All data are presented as mean ± SEM of biologically independent samples, analyzed using two-tailed t-tests. *p<0.05, **p<0.01, ***p<0.001.
