Extended Data Fig. 10: Analysis of β cells after injuries by inducible Ins2-DreER;H11-rox-tdT system.
From: Pre-existing beta cells but not progenitors contribute to new beta cells in the adult pancreas
a, A schematic diagram illustrating the experimental design to test for β cell neogenesis during pregnancy in the inducible system. 6 weeks old mice (only female mice were used) were treated with Tamoxifen for 3 times. After washout for 2 weeks and Pregnancy for 15.5 days, mice were euthanized (Pregnancy group, n = 5; Sham group, n = 5). b, Immunostaining for tdT and Ins on pancreatic slides collected from Ins2-DreER;H11-rox-tdT during pregnancy (Preg15.5). c, Quantification of β cells expressing tdT in the indicated mice. Numbers of investigated mice were as follows: Pregnancy group, n = 5; Sham group, n = 5, Data are mean ± SEM, n.s., not significant (unpaired, two-sided, Student’s t-test). d, A schematic diagram illustrating the experimental design to test for β cell neogenesis after pancreatic ductal ligation (PDL). 6 weeks old mice (both male and female mice were used) were treated with Tamoxifen for 3 times. After washout for 2 weeks and PDL injury for 2 weeks, mice were euthanized (PDL group, n = 5; Sham group, n = 5). e, Immunostaining for tdT and Ins on pancreatic slides collected from Ins2-DreER;H11-rox-tdT after PDL. f, Quantification of β cells expressing tdT in the indicated mice. Numbers of investigated mice were as follows: PDL group, n = 5; Sham group, n = 5, Data are mean ± SEM, n.s., not significant (unpaired, two-sided, Student’s t-test). g, A schematic diagram illustrating the experimental design to test for β cell neogenesis after streptozocin (STZ)-induced injury. 6 weeks old mice (both male and female mice were used) were treated with Tamoxifen for 3 times. After washout for 2 weeks and STZ injury for 2 weeks, mice were euthanized (STZ group, n = 5; Sham group, n = 5). h, Immunostaining for tdT and Ins on pancreatic slides collected from Ins2-DreER;H11-rox-tdT after streptozocin (STZ)-induced injury. (i) Quantification of β cells expressing tdT in the indicated mice. Numbers of investigated mice were as follows: STZ group, n = 5; Sham group, n = 5, Data are mean ± SEM, n.s., not significant (unpaired, two-sided, Student’s t-test). Scale bars, yellow, 1 mm; white, 100 μm. Each image is representative of 5 individual biological samples.
