Extended Data Fig. 8: Proline availability regulates transcript levels of multiple nucleotide biosynthesis genes. (Data supporting Fig. 7).
From: Mitochondrial NADP+ is essential for proline biosynthesis during cell growth
a,b, Transcript levels are shown for the indicated nucleotide biosynthesis genes from isogenic ΔNADK2 HeLa (a), or K562 (b) cells stably expressing either empty vector or NADK2 grown in DMEM supplemented with 10% serum in the presence or absence of proline (0.2 mM). Data are presented as the mean ± SEM from =3 of biologically independent samples that were each measured in technical triplicates. *P < 0.05, **P < 0.01 for comparisons calculated using one-way ANOVA test and Tukey’s post-hoc test.
