Extended Data Fig. 5: Assessment of UCP1/SUCNR1KO during WD and succinate drinking water experiments.
From: UCP1 governs liver extracellular succinate and inflammatory pathogenesis
a, Representative cytofluorimetric dot plots for indicated immune cell populations from livers of WT, UCP1KO and UCP1/SUCNR1KO mice following 14 weeks on WD. b, Relative gene expression in WT, UCP1KO, and UCP1/SUCNR1KO livers following 14 weeks WD feeding (n = 10 except Il6, Nos2 n = 9 in WT and Nos2 n = 9 in UCP1/SUCNR1KO). c, Fraction of CD45+ cells for each indicated gated cell population from livers of WT, UCP1KO and UCP1/SUCNR1KO mice following 14 weeks on WD (n = 9 except CD8 n = 7 in UCP1/SUCNR1KO). d-h, Protein abundance differences of annotated pathways proteins or HSC activation proteins between vehicle and 1.5% succinate-treated UCP1KO liver following 6 weeks HFD (n = 3). Data represent fold over 0%. i, Sirius red staining observed in liver harvested from mice following 14 weeks WD feeding (upper panels 20x magnification, scale bars 100 μm; middle panels, 10x magnification, scale bars 200 μm, lower panels, 4x magnification, scale bars 200 μm; n = 4 biological replicates/genotype imaged). j, Relative abundance of hydroxyproline in plasma following 14 weeks on WD WT (n = 10). *P < 0.05, **P < 0.01, ***P < 0.001. (two-tailed Student’s t-test for pairwise comparisons, one-way ANOVA for multiple comparisons involving independent variable). Data are mean ± s.e.m. Assessments of UCP1/SUCNR1KO mice were performed simultaneously with WT and UCP1KO as depicted in Fig. 2 and Extended Data Fig. 2, so WT and UCP1KO presentations in this figure are from the same underlying data reported in Fig. 2 and Extended Data Fig. 2. See source data for precise p values.
