Extended Data Fig. 4: The UPR is necessary for HG-induced β-cell proliferation (related to Fig. 7).
From: In vivo screen identifies a SIK inhibitor that induces β cell proliferation through a transient UPR
(a) mRNA levels of genes belonging to the ATF6, IRE1, or PERK pathways after treatment of mouse islet cells with DMSO or HG in low glucose medium. N = 3 independent experiments. Two-sided student’s t-test. **P = 0.001 for Sec24d, ***P ≤ 0.001 for Pdia4. (b) In vivo effect of HG (10 mg/kg) on UPR activation in wild-type mice. Left panel: Representative images of islets from control and HG-treated mice after 14 h of treatment. Scale bar: 20 µm. In blue: DAPI; in green: insulin; in red: BIP. The arrows show BIP+ β-cells. Right panel: Quantification of the percentage of mouse β-cells expressing BIP per islet. Data are represented as the mean ± SEM. Two-sided student’s t-test. **P = 0.0032. N = 123 islets from 6 HG-treated mice and 131 islets from 6 control-treated mice.
