Extended Data Fig. 5: TPI1 S117 phosphorylation promote TPI1 nucleus translocation (Related to Fig. 3). | Nature Metabolism

Extended Data Fig. 5: TPI1 S117 phosphorylation promote TPI1 nucleus translocation (Related to Fig. 3).

From: Nuclear dihydroxyacetone phosphate signals nutrient sufficiency and cell cycle phase to global histone acetylation

Extended Data Fig. 5

a, Confirmation of homozygous knock-in of TPI1 and CDK2 mutants. Sequencing results of S117A and S117D of TPI1 mutants knock-in and T160A and T160D of CDK2 mutants knockin were shown. b, TPI1 and CDK2 mutations altered cell cycle progression. FACS results of HeLa cells and homozygous knockin S117A and S117D of TPI1 and T160A and T160D of CDK2 were shown, the gating strategy of flow cytometry was shown in Extended Data Fig. 6b. c, TPI1 S117 mutations affected cell growth. The growth of HeLa, TPI1S117A and TPI1S117D knockin HeLa cells were compared. n = 3 biologically independent samples (P = 0.0089, 0.0412). d, S117D and S117A retained catalytic activities. Specific activities were determined for TPI1 (set as 100% arbitrarily), S117D and S117A that were expressed and purified from HEK293T. n = 4 biologically independent experiments (P =0.5734, 0.0798).

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