Extended Data Fig. 6: Synchronizing and releasing of CDK2 knockdown cells (related to Fig. 4). | Nature Metabolism

Extended Data Fig. 6: Synchronizing and releasing of CDK2 knockdown cells (related to Fig. 4).

From: Nuclear dihydroxyacetone phosphate signals nutrient sufficiency and cell cycle phase to global histone acetylation

Extended Data Fig. 6

a, CDK2 knockdown HeLa cells were synchronized to G1/S boundary with DTB. The released cells were collected at different time and the percentage of cells at different cell cycle phases were determined by flow cytometry (upper) and quantified (middle and bottom), the gating strategy of flow cytometry was shown in Extended Data Fig. 6b. b, Gating strategy of flow cytometry for normal HeLa cells(upper) and DTB Block Released 0hr HeLa cells(bottom).

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