Extended Data Fig. 7: Nutrient regulates DHAP and mTORC1 (related to Fig. 5).
a, Nutrient failed to alter histone acetylation in TPI1 S117 mutants knockin cells. The responses of histone acetylation levels to nutrient were detected in HeLa and S117A and S117D knockin HeLa cells. b-c, Nutrient failed to alter nuclear acetate and DHAP levels and histone acetylation levels in TPI1 S117 mutants knockin cells. The nuclear acetate and DHAP levels (b) and histone acetylation levels (c) were detected in HeLa and S117A and S117D knockin HeLa cells that were cultured under absence and presence of glucose and glutamine in the culture media. n = 3 biologically independent samples (P = 0.0015, 0.0009, 0.3713, 0.5402, 0.0735, 0.1577). d-e, Activating mTORC1 abrogated nutrient to regulate nuclear acetate and DHAP levels and histone acetylation levels. The nuclear acetate and DHAP levels (d) and histone acetylation levels (e) were detected in wildtype and NPRL3 knockout HeLa cells cultured in the absence and presence of glucose or glutamine. n = 3 biologically independent samples (P = 5.72e-6, 0.0007, 3.95e-5, 0.0003, 0.5726, 0.5161, 0.1373, 0.5944). f, Rapamycin failed to alter histone acetylation in TPI1 S117 mutants knockin cells. The responses of histone acetylation levels to rapamycin were detected in HeLa and S117A and S117D knockin HeLa cells. g, Raptor interacted with CDK2. Raptor and CDK2 were co-expressed in HeLa cells, co-immunoprecipitation was carried out to detect interaction between them. h. CDK2 T160 mutations affect cell growth. The growth of HeLa, CDK2T160A and CDK2T160D knockin HeLa cells were compared. n = 3 biologically independent samples.
