Extended Data Fig. 5: Mitochondrial function in wild-type, Bmpr1a−/− and 4n mESCs.
a-d, Metabolic flux analysis of wild-type and Bmpr1a−/− mESCs. OCR profile and metabolic parameters assessed during the mitochondria stress test performed in pluripotency conditions (a). ECAR profile and metabolic parameters assessed during the glycolysis stress test performed in pluripotency conditions (b). Metabolic parameters from the mitochondria stress test found to be similar between wild-type and Bmpr1a−/− mESCs during differentiation – day 3 (c). Metabolic parameters from the glycolysis stress test found to be similar between wild-type and Bmpr1a−/− mESCs during differentiation – day 3 (d). Data obtained from 3 (a,b) or 5 (c,d) independent experiments, with 5 replicates per cell type in each assay. e-f, Analysis of mitochondrial membrane potential (Δψm) in defective mESCs maintained in pluripotency conditions, in separate or co-culture. Representative histograms of TMRM fluorescence and quantification for wild-type and Bmpr1a−/− (e) and wild-type and 4n (f). g, Analysis of mitochondrial ROS in wild-type and Bmpr1a−/− mESCs undergoing differentiation in separate or co-culture: representative histograms of mitoSOX Red fluorescence and quantification of the percentage of mitoSOX positive cells. Data shown as mean ± SEM from 3 (e-f) or 5 (g) independent experiments. See methods for details on statistical analysis.
