Extended Data Fig. 7: Analysis of SNPs in mtDNA in epiblast cells.

a-e, mtDNA heteroplasmy (plotted as Heteroplasmy = 1- frequency of most common allele) in epiblast cells from CI-treated embryos for five positions within the mt-Rnr2 gene. All these positions have an heteroplasmy that increases with the cells’ losing scores in a statistically significant way - the adjusted p-values are indicated at the top of each plot. f-k, The variation in the heteroplasmy across the CI-treated cells is not due to a batch effect for the 6 significant positions within the mt-Rnr1 gene. The number of cells analysed per cluster (and batch) is as follows: number of cells in Normal Epiblast :42 (1),16 (2),18 (3),0 (4),2 (5); number of cells in Intermediate: 42 (1), 28 (2), 28(3), 12 (4), 5 (5); number of cells in Loser Epiblast: 22 (1), 15(2), 20 (3), 2 (4), 7 (5). l, Correlation between the mtDNA heteroplasmy at all the statistically significant positions, six within the gene mt-Rnr1 and five within the gene mt-Rnr2. m, Schematic representation of the mitochondrial genome showing in red the positions that passed our filtering based on coverage and were considered for the heteroplasmy analysis. Only the genes that include these positions are indicated. See methods for details on statistical analysis.