Extended Data Fig. 8: Peripheral irisin reduces glia activation in transgenic mouse models of AD.
From: Exercise hormone irisin is a critical regulator of cognitive function
APP/PS1 mice were injected with AAV8-GFP or AAV8-irisin-FLAG via the tail vein. a, Expression of synaptic plasticity genes in hippocampus derived from normalized read counts of RNA-seq analysis (GFP n = 5, irisin n = 5), b, MSD ELISA for Abeta40 peptide in cortex soluble fraction, c, MSD ELISA Abeta42 peptide in cortex soluble fraction (GFP n = 9, irisin n = 5). d and e, Representative immunofluorescence confocal images of hippocampus, GFAP (green), Iba-1 (red), 3D6 (Alexa 647). Scale bar 200 μm (d) and quantification of plaque burden in hippocampus (e) (GFP n = 7, irisin n = 5). f, Quantification of plaque burden cortex (GFP n = 5, irisin n = 3). g, Quantification of total BrdU+ cells (GFP n = 7, irisin n = 5). Two-way ANOVA (a), Two-tailed t-test (b, c, e-g). n.s.= not significant, data are represented as mean ± SEM of biologically independent samples.
