Extended Data Fig. 9: Irisin binds αV/β5 integrin receptor on astrocytes in adult hippocampal neural stem cells cultures.
From: Exercise hormone irisin is a critical regulator of cognitive function
a, Two-color dSTORM images of integrin αV/β3 (left) and αV/β5 (right) (green) and irisin-FLAG (red) molecules. Scale bar 5 μm (irisin-FLAG-αV/β3 n = 7, irisin-FLAG-αV/β5 n = 10), b and c, Itgav gene expression (b) and Itgb5 gene expression (c) in the murine dentate gyrus from Linnarsson lab database (https://linnarssonlab.org/dentate/)59. d, QPCR analysis of mRNA expression of Map2, Dcx, Gfap, and Aif1 in the dentate gyrus (n = 6) and neurons differentiated from adult hippocampal stem cells (n = 8 from two independent experiments). Ct: cycle threshold value. Data are represented as mean ± SEM of biologically independent samples.
