Extended Data Fig. 2: An examination of mesenteric lymphatic dysfunction in 15-17 week old CFD fed db/db mice in comparison to C57BL/6 mice fed a CFD or HFD for 6, 15, 23 or 32 weeks from 6-7 weeks of age.
a, Total body weight. Mean ± s.e.m for n = 9, 9, 5. b, Representative immunofluorescence images of the mesenteric lymphatic vessels (LYVE-1 (green)) in VAT of db/db and C57BL/6 mice. Note white arrow indicates the direction of lymph flow, arrow heads indicate point of vessel branching. Scale bar, 500 μm. c, Quantification of mesenteric lymphatic vessel branching in VAT of mice. Mean ± s.e.m for n = 4 or n = 5 mice for week 6, n = 6 or n = 7 mice for week 15, n = 4 or n = 5 mice for week 23, n = 4 or n = 5 mice for week 32 and n = 5 for db/db mice. d, Representative images of Evans blue dye passage through mesenteric lymphatic vessels and leakage to VAT 10 min after intramucosal dye injection to CFD and HFD fed C57BL/6 mice and db/db mice. Scale bar, 5 mm. White circle highlights a site of lymph leakage. e, Area under the curve (AUC) of the Evan’s blue dye intensity plots. Mean ± s.e.m., n = 7, 7 and 5 mice. f, Total cells in mesenteric lymph of mice fed CFD or HFD for 6, 15, 23, 32 weeks or db/db mice fed CFD. Mean ± s.e.m for n = 5 or n = 4 mice for week 6, n = 8 or n = 9 mice for week 15, n = 6 or n = 6 mice for week 32 and n = 3 for db/db mice. g, Weight of subcutaneous adipose tissue (SAT, inguinal adipose tissue depot) and mesenteric adipose tissue (MAT). Note comparisons across CFD, HFD and db/db mice in (b, d, e, g) are made between C57BL/6 mice fed CFD or HFD for 23 weeks and db/db mice age 15-17 weeks. Statistical differences, *p < 0.05, **p < 0.01, ***p < 0.005, or ****p < 0.0001 from (a, e) one-way ANOVA, (c) two-tailed Mann-Whitney test for week 6, 23 and 32 data, two-tailed Student’s t test was used for week 15 data, one-way ANOVA was used for comparison between db/db mice and week 23 and week 32 data, (f) a two-tailed Student’s t-test (except one-way ANOVA was used for comparison between db/db mice vs week 6 data), (g) two-way ANOVA. P values and details of the statistical testing are provided as source data.
