Extended Data Fig. 3: Body weight, and composition and effects of mesenteric lymph from Sprague-Dawley rats fed CFD or HFD for 6-9 weeks.
a, Body weight and b, TG concentration. Mean ± s.e.m for n = 4, 4 (HFD) rats. c, Heatmap analysis of lipidomics data of lymph from HFD mice and rats, expressed as mean peak intensity relative to CFD mice and rats, respectively. Blue indicates decreased abundance, red indicates increase and yellow signifies no change. n = 4, 4 mice/rats. d-e, PGE2 and VEGFc concentration in mesenteric lymph of rats. Mean ± s.e.m, n = 4, 4 rats. Statistical differences, *p < 0.05 from two-tailed Mann-Whitney test. f, Real time PCR analysis of Fabp4, Lepr, Gapdh, Pparg, Pnpla2, and Il6 mRNA expression in 3T3-L1 adipocytes treated with control media (Ctrl) or 0.5 or 2% v/v CFD-lymph in media. Mean ± s.e.m. for n = 3 from N = 1 experiment. g, Lipolysis in mature 3T3-L1 adipocytes (from glycerol release) in basal and forskolin-stimulated conditions after treatment with control media (Ctrl) or 2% lymph chylomicron (CM), VLDL or lipid-free protein fraction (supernatant). Mean ± s.e.m. for n = 3 except Ctrl (n = 9 (basal) and n = 6 (forskolin)) from N = 1 experiment. Statistical differences, *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001 from Two-Way ANOVA. h, 14C-2-deoxyglucose (2DG) uptake into SPF3 human adipocytes in basal and insulin-stimulated conditions after treatment with control media or 2% HFD rat lymph. Mean ± s.e.m. for n = 8, 8 (basal) and n = 6, 7 (insulin) replicates from N = 3 experiments. Statistical differences, *p < 0.05 from two-way ANOVA. P values and details of the statistical testing are provided as source data.
