Extended Data Fig. 7: Absolute concentration of L-2-HG, and concentration titration for treating embryos.
From: Metabolic remodelling during early mouse embryo development
(a) The extracted-ion chromatogram of L-2-HG and D-2-HG standards (concentration 100 nM). It shows clear separation between these two enantiomers. (b) The absolute concentration of L-2-HG and D-2-HG in MII, zygote, 2-cell and blastocyst embryos. (c) Reported absolute concentration of 2-HG in various cell lines, tumor and tissues. (d) Schematic illustration of Tunel experimental approach. Zygotes were collected 16 hours after injection of hCG and cultured in KSOM with different concentration L-2-HG. (e) Tunel assay was performed at different stage embryos with different concentration. Embryos were cultured in KSOM with L-2-HG at 0 mM, 0.15 mM, 0.3 mM, 0.45 mM and 0.6 mM. Representative images of full z-series confocal max projections of embryos in three independent experiments are shown. The scale bar is 50 µm.
