Extended Data Fig. 10: 5-IP7 clamps Syt7 in a Ca2+-releasable manner to regulate Syt7-PIP2 interaction. | Nature Metabolism

Extended Data Fig. 10: 5-IP7 clamps Syt7 in a Ca2+-releasable manner to regulate Syt7-PIP2 interaction.

From: 5-IP7 is a GPCR messenger mediating neural control of synaptotagmin-dependent insulin exocytosis and glucose homeostasis

Extended Data Fig. 10: 5-IP7 clamps Syt7 in a Ca2+-releasable manner to regulate Syt7-PIP2 interaction.

(a) FRET assay measuring the binding of NBD-labelled Syt7 to Rhodamine-PE labelled liposome, in the presence of 0.5 mM MgCl2 and increasing concentrations of IP6 (middle panel) or 5-IP7 (right panel). In this experiment, 1 µM IANBD-Syt7, 100 µM (total lipids) liposome, 25 µM CaCl2, 0.5 mM MgCl2, and indicated concentrations of IP6/5-IP7 were mixed and measured in 1-cm cuvette at 25 °C. Data points are presented as means ± s.d. (n = 3, technical replicates). (b) Concentration-dependent effect of 5-IP7 and IP6 on the interaction between Ca2+ (2 μM) and Fura2 dye (2 μM).

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