Extended Data Fig. 1: PKC and PKD phosphorylates IP6K1 at S121 and S118, respectively. | Nature Metabolism

Extended Data Fig. 1: PKC and PKD phosphorylates IP6K1 at S121 and S118, respectively.

From: 5-IP7 is a GPCR messenger mediating neural control of synaptotagmin-dependent insulin exocytosis and glucose homeostasis

Extended Data Fig. 1: PKC and PKD phosphorylates IP6K1 at S121 and S118, respectively.

(a) Phos-tag gel electrophoresis of immunoprecipitated IP6K1 reveals a slower migrating species that is absent under normal PAGE. (b-c) Mass-spectrum of immunoprecipitated IP6K1 reveals phosphorylation at S118/S121 (b) and S127 (c). (d) Sequence alignments of the three human IP6K isoforms. The poorly-conserved unstructured region is marked by red rectangles. (e) The disorder tendency of IP6K1 determined by IUPred (http://iupred.enzim.hu/). IDR: intrinsic disordered region. (f) Phosphorylation status of IP6K1 and its mutants examined with antibodies specifically recognizing phosphorylated PKC and PKD substrates. (g-h) In vitro phosphorylation of GST-IP6K1 purified from HEK293 cells by purified PKCβ (g) and PKD (h). GST-IP6K1 overexpressed in a 10-cm plate was pull-down, washed extensively and then incubated with commercial PKCβ and PKD.

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